We show that this process relies on CRISPR components; is sequence-specific; and, upon simultaneous introduction of multiple gRNAs, can effect multiplex editing of target loci. Our results establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
National Center for Biotechnology Information , U.
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Epub Jan 3. New tool for genome surgery. Images from this publication.
See all images 2 Free text. Any genomic sequence of the form GN 20 GG can, in principle, be targeted. Representative FACS plots and microscopy images of the targeted cells are depicted below.
F Successfully targeted clones of T cells were selected with puromycin for 2 weeks. Supplemental Content Full text links.